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The multispecific cradle polypeptide according to claim 72, wherein the monomer cradle connected by a polypeptide linker sequence. The cradle polypeptide according to claim 20, wherein the length of the FG loop is about 5 or 6 residues. Antibody EngineeringiMiniantibodies “antibody engineering: The polynucleotide according to claim 91, wherein the polynucleotide is an expression construct. The library of any one of claims, wherein said cradle library to bind preselected molecules Gerba.

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The library of any one of claims, wherein said cradle polypeptide comprises at least one loop region to at least one amino acid deletion I. The cradle polypeptide according to claim 23, wherein the position of the FG loop I is Gly residues, the position of the FG loop 2 is Leu, Val or Ile residue, the position of the FG loop 3 is charged or polar residues, the position of the FG loop 4 is Pro residue, the position of the FG loop is Gly residues 5, 6 and the position of the FG loop is a polar residue.

C sheet; Figure 4B: The cradle polypeptide according to claim 17, wherein the length of the CD loop of about residues.

Computer-aided FnIII cradle library building. The polynucleotide according to claim 92, wherein said expression construct capable of expressing encoded polypeptides in bacteria, yeast or mammalian systems. Ssssss dddddd Ssssss dddddd.

Based on the cradle domain polypeptide of fibronectin type Jonitor FnIIIsaid cradle comprises at least one loop region polypeptide and at least one unsubstituted ring region of one or more amino acids. FnIII cradle library synthesis. The method of claimwherein the length of the FG loop is 6 residues. Expression and screening system.

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CN103403027A – Fibronectin cradle molecules and libraries thereof – Google Patents

Effect slice positions 1,3,4,5,6,7,8 and 9. The polypeptide of any one of the preceding claims, characterized in that the length of the FG loop is about 1 to 10 residues. Interestingly, the two acidic residues are conserved in strong ySUMO cradle molecule, indicate that a combination of these contacts dependence may not be so strong.

The cradle library according to claim 79, wherein said cradle polypeptide comprises at least one loop region to at least two amino acid insertions.

The cradle library according to claim 82, wherein said cradle polypeptide comprises v775f least one loop region to at least two amino acid deletion. The cradle polypeptide according to claim 21, wherein the length of the FG loop is 6 residues. The cradle library according to minitor 80, wherein said cradle polypeptide comprises at least one loop region of about amino acid insertions.

Struct Funct Genet Antibody EngineeringiMiniantibodies “antibody engineering: The cradle library according to any one of the preceding claims, characterized in that, said cradle comprising two loop regions in a polypeptide of at least two amino acid deletion.

Generation of antibodies to cell-surface receptors and cancer-associated proteins including egfr family members. The library of any one of claims, wherein said cradle library to bind preselected molecules Gerba. Methods for identifying nucleic acid molecules encoding poly peptides that interact with target molecules. May include, but not limited to, ,team Biological Chem A polynucleotide encoding a claim.

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The cradle polypeptide according to claim 21, wherein the length of the FG loop is v75r residues. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity. The method of any one of the preceding claims, wherein said step ii into the ring and longitudinal length of about 1 to 10 residues of the FG loop.

The multispecific cradle polypeptide according to claim 72, wherein the monomer cradle connected by a polypeptide linker sequence. Production of gene and protein analogs sdi synthetic gene design using double stranded synthetic oligonucleotides.

Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by ad surface display: Dooooo Haaaaa Dooooo Haaaaa.

The cradle polypeptide according to claim 18, wherein the length of the CD ring is 5 residues. Polo cartridgeproline-rich binding region e.

The polypeptide of any one of the preceding claims, characterized in that the length of the CD loop is about residues.